MR guidance of targeted injections into border and core of scarred myocardium in pigs

Radiology. 2006 Aug;240(2):419-26. doi: 10.1148/radiol.2402051086. Epub 2006 Jun 26.

Abstract

Purpose: To use (a) dysprosium-based contrast agent (sprodiamide) to confirm the site of myocardial injection and (b) T1-enhancing magnetic resonance (MR) contrast media to mark the myocardial target and T2*-enhancing contrast media to demonstrate injection sites in the margins or core of infarction on delayed contrast-enhanced images.

Materials and methods: Approval of the institutional committee on animal research was obtained. A phantom and six pigs subjected to chronic infarction (8 weeks) underwent MR-guided experiments. At inversion-recovery gradient-echo imaging, gadoterate meglumine (0.1 mmol/kg) was intravenously administered to delineate scar tissue. A catheter fitted with multiple receiver coils was used to visualize catheter navigation and injection sites. A steady-state free precession (balanced fast field-echo) sequence was used for MR fluoroscopy. A high-resolution multiphase balanced gradient-echo cine MR sequence was used after intramyocardial deposition of sprodiamide. The border and core of scarred myocardium were characterized histopathologically. The 95% confidence interval (CI) was used to demonstrate the range, extent of hyperenhanced and hypoenhanced regions after contrast media administration.

Results: In the phantom and in vivo, the actively guided catheter produced a high signal intensity at the terminal portion of the shaft and tip. Scarred myocardium was recognized as a bright region on gadoterate meglumine-enhanced images. Intramyocardial injection of sprodiamide caused local and persistent signal intensity loss, and the extent was volume dependent on balanced fast field-echo and T2-weighted turbo spin-echo images. At 5 minutes after administration of 0.2, 0.4, and 0.6 mL of sprodiamide, the 95% CIs of the extents of the hypoenhanced regions were 0.08%, 0.23%; 0.27%, 0.51%; and 0.46%, 0.70%, respectively, of left ventricular (LV) surface area (P < .05, paired t test). Failure of intramyocardial injection was confirmed by a brief signal loss of LV chamber blood.

Conclusion: Sprodiamide allows visualization of injection sites within enhanced infarction. A catheter with integrated receiver coils aided in effective catheter guidance and precise intramyocardial injection.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Contrast Media / administration & dosage*
  • Image Enhancement / methods
  • Injections
  • Magnetic Resonance Imaging / methods*
  • Meglumine / administration & dosage*
  • Myocardial Infarction / pathology*
  • Organometallic Compounds / administration & dosage*
  • Pentetic Acid / administration & dosage
  • Pentetic Acid / analogs & derivatives*
  • Phantoms, Imaging
  • Swine

Substances

  • Contrast Media
  • Organometallic Compounds
  • sprodiamide
  • Meglumine
  • Pentetic Acid
  • gadoterate meglumine