Cytohistology, colposcopy and in situ hybridization in cervical preneoplastic lesions: a correlative study

Int J Gynaecol Obstet. 1991 Aug;35(4):323-6. doi: 10.1016/0020-7292(91)90665-r.

Abstract

Formalin-fixed, paraffin-embedded cervical tissues from 106 patients which exhibited a colposcopically atypical transformation zone, were examined for the presence of HPV DNA by in situ hybridization using biotin-labeled HPV 6/11 and 16/18 DNA probes. From the comparative study between histologic, cytologic, colposcopic findings and virologic results it is confirmed that HPV 6/11 DNA is detected more frequently in the less severe lesions, whereas HPV 16/18 DNA is predominantly found in the most severe dysplastic lesions.

Publication types

  • Clinical Trial

MeSH terms

  • Cervix Uteri / cytology
  • Cervix Uteri / microbiology
  • Cervix Uteri / pathology
  • Chi-Square Distribution
  • Colposcopy
  • DNA Probes, HPV*
  • DNA, Viral / analysis
  • Female
  • Humans
  • Molecular Probe Techniques
  • Papillomaviridae / genetics
  • Papillomaviridae / isolation & purification*
  • Uterine Cervical Dysplasia / microbiology
  • Uterine Cervical Dysplasia / pathology*
  • Vaginal Smears

Substances

  • DNA Probes, HPV
  • DNA, Viral