The human IgA2 subclass has two allotypes A2m(1) and A2m (2). Using the RFLP technique, we determined the A2m(2) frequency in a sample of 29 tunisian individuals. In accordance with Lefranc and all. [6], the presence of a polymorphic EcoRI site of the A2m(2) allele was confirmed in our RFLP study whereas no BamHI nor HindIII polymorphisms were shown. Among the 29 cases studies, 11 were heterozygous A2m2-1, 17 homozygous A2m1-1 and 1 homozygous A2m2-2. The A2m2 allele frequency in the Tunisian population (0,22) is intermediate between the European frequency and the African one.