[Experience in using the latent activity of leukocytic lactate dehydrogenase isoenzymes for the integral estimate of the level of free radical oxidation in patients with neurotic disorders]

Klin Lab Diagn. 2006 Jun:(6):16-20.
[Article in Russian]

Abstract

The aggregatory properties of a leukocytic homogenate were studied by analyzing the activity of its lactate dehydrogenase (LDH) isoenzymes from patients with neurotic disorders on admission and during treatment. As a parameter reflecting the aggregatory properties of the leukocytic homogenate, the latent activity of LDH isoenzymes was studied. On admission, the patients were shown to have a lower latent activity, which restored during treatment to the control values, than in the control group. There was also a synchronous pattern of a change in the osmotic stability of red blood cells with the latent activity of leukocytic LDH isoenzymes in the treated patients. It is obvious that latent activity values reflect the level of free radical oxidation in the body. For detailed testing of the aggregatory properties of a cellular lysate, the trends in the latent activity of LDH isoenzymes were examined, which failed to reveal an unambiguous recovery of the observed parameters during therapy. Based on the findings, the author discusses whether this method can be used to analyze the time course of changes in a psychopathological process and to predict its outcome.

Publication types

  • English Abstract

MeSH terms

  • Adult
  • Female
  • Free Radicals / metabolism*
  • Humans
  • Isoenzymes / metabolism
  • L-Lactate Dehydrogenase / metabolism*
  • Leukocytes / enzymology*
  • Leukocytes / pathology
  • Male
  • Middle Aged
  • Neurotic Disorders / enzymology*
  • Neurotic Disorders / therapy
  • Oxidation-Reduction

Substances

  • Free Radicals
  • Isoenzymes
  • L-Lactate Dehydrogenase