Comparison of the LCx human immunodeficiency virus (HIV) RNA quantitative, RealTime HIV, and COBAS AmpliPrep-COBAS TaqMan assays for quantitation of HIV type 1 RNA in plasma

Vincent Foulongne; Brigitte Montes; Marie-Noelle Didelot-Rousseau; Michel Segondy

doi:10.1128/JCM.00341-06

Comparison of the LCx human immunodeficiency virus (HIV) RNA quantitative, RealTime HIV, and COBAS AmpliPrep-COBAS TaqMan assays for quantitation of HIV type 1 RNA in plasma

J Clin Microbiol. 2006 Aug;44(8):2963-6. doi: 10.1128/JCM.00341-06.

Authors

Vincent Foulongne¹, Brigitte Montes, Marie-Noelle Didelot-Rousseau, Michel Segondy

Affiliation

¹ Division of Virology, Department of Infectious Diseases, Montpellier University Hospital, 34295 Montpellier, France.

Abstract

The LCx human immunodeficiency virus (HIV) RNA Quantitative, RealTime HIV, and COBAS AmpliPrep-COBAS TaqMan assays for HIV type 1 (HIV-1) were compared for their abilities to quantitate HIV-1 RNA in plasma. High degrees of correlation and agreement were observed between the assays. Differences in HIV-1 RNA levels according to HIV-1 subtypes did not reach statistical significance.

Publication types

Comparative Study
Evaluation Study

MeSH terms

HIV Infections / virology*
HIV-1 / genetics
HIV-1 / isolation & purification*
Humans
Polymerase Chain Reaction / methods*
RNA, Viral / blood*
Reagent Kits, Diagnostic
Viral Load*

Substances

RNA, Viral
Reagent Kits, Diagnostic