Generation of a reporter cell line for detection of infectious varicella-zoster virus and its application to antiviral studies

Antimicrob Agents Chemother. 2006 Sep;50(9):3142-5. doi: 10.1128/AAC.00342-06.

Abstract

To simplify the titration of infectious varicella-zoster virus (VZV), we generated a reporter cell line that produced luciferase in a dose-dependent manner upon infection with cell-free VZV. A few VZV-infected cells were detectable by coculturing with the cell line. We demonstrated the usefulness of the cell line for antiviral studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antiviral Agents / pharmacology*
  • Base Sequence
  • Cell Line / enzymology*
  • Cell Line / virology*
  • Genes, Reporter / genetics
  • Herpesvirus 3, Human / drug effects
  • Herpesvirus 3, Human / genetics*
  • Luciferases / biosynthesis
  • Luciferases / genetics
  • Microbial Sensitivity Tests / methods*
  • Molecular Sequence Data
  • Open Reading Frames
  • Promoter Regions, Genetic

Substances

  • Antiviral Agents
  • Luciferases