Abstract
We evaluated a highly sensitive quantitative real-time one-step reverse transcription-PCR (RT-PCR) for detection of human cytomegalovirus pp67 transcripts in monitoring of solid-organ transplant recipients. Results were compared with those of pp65 antigen testing and quantitative DNA-PCR. Due to a low clinical sensitivity, the pp67 RT-PCR was not able to replace these assays.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Cytomegalovirus / genetics
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Cytomegalovirus / isolation & purification
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Cytomegalovirus Infections / diagnosis*
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Cytomegalovirus Infections / virology
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DNA, Viral / analysis
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Humans
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Organ Transplantation / adverse effects*
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Phosphoproteins* / blood
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Polymerase Chain Reaction
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Viral / genetics
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RNA, Viral / metabolism
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Viral Matrix Proteins* / blood
Substances
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DNA, Viral
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Phosphoproteins
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RNA, Messenger
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RNA, Viral
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Viral Matrix Proteins
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cytomegalovirus matrix protein 65kDa
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pp67 protein, human cytomegalovirus