Use of a HpaII-polymerase chain reaction assay to study DNA methylation in the Pgk-1 CpG island of mouse embryos at the time of X-chromosome inactivation

Mol Cell Biol. 1990 Sep;10(9):4987-9. doi: 10.1128/mcb.10.9.4987-4989.1990.

Abstract

A HpaII-PCR assay was used to study DNA methylation in individual mouse embryos. It was found that HpaII site H-7 in the CpG island of the X-chromosome-linked Pgk-1 gene is less than or equal to 10% methylated in oocytes and male embryos but becomes 40% methylated in female embryos at 6.5 days; about the time of X-chromosome inactivation of the inner cell mass.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • DNA / genetics*
  • Deoxyribonuclease HpaII
  • Deoxyribonucleases, Type II Site-Specific
  • Dinucleoside Phosphates*
  • Embryo, Mammalian / physiology*
  • Female
  • Male
  • Methylation
  • Mice
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Phosphoglycerate Kinase / genetics*
  • Polymerase Chain Reaction
  • Restriction Mapping
  • X Chromosome*

Substances

  • Dinucleoside Phosphates
  • Oligonucleotide Probes
  • cytidylyl-3'-5'-guanosine
  • DNA
  • Phosphoglycerate Kinase
  • Deoxyribonuclease HpaII
  • Deoxyribonucleases, Type II Site-Specific