The RAC binding domain/IRSp53-MIM homology domain of IRSp53 induces RAC-dependent membrane deformation

J Biol Chem. 2006 Nov 17;281(46):35347-58. doi: 10.1074/jbc.M606814200. Epub 2006 Sep 25.

Abstract

The concave surface of the crescent-shaped Bin-amphiphysin-Rvs (BAR) domain is postulated to bind to the cell membrane to induce membrane deformation of a specific curvature. The Rac binding (RCB) domain/IRSp53-MIM homology domain (IMD) has a dimeric structure that is similar to the structure of the BAR domain; however, the RCB domain/IMD has a "zeppelin-shaped" dimer. Interestingly, the RCB domain/IMD of IRSp53 possesses Rac binding, membrane binding, and actin filament binding abilities. Here we report that the RCB domain/IMD of IRSp53 induces membrane deformation independent of the actin filaments in a Rac-dependent manner. In contrast to the BAR domain, the RCB domain/IMD did not cause long tubulation of the artificial liposomes; however, the Rac binding domain caused the formation of small buds on the liposomal surface. When expressed in cells, the Rac binding domain induced outward protrusion of the plasma membrane in a direction opposite to that induced by the BAR domain. Mapping of the amino acids responsible for membrane deformation suggests that the convex surface of the Rac binding domain binds to the membrane in a Rac-dependent manner, which may explain the mechanism of the membrane deformation induced by the RCB domain/IMD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Cell Line
  • Cell Membrane / metabolism*
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Humans
  • Liposomes
  • Models, Molecular
  • Nerve Tissue Proteins / metabolism*
  • Protein Binding
  • Protein Conformation
  • Protein Structure, Tertiary
  • rac GTP-Binding Proteins / metabolism*

Substances

  • BAIAP2 protein, human
  • Liposomes
  • Nerve Tissue Proteins
  • rac GTP-Binding Proteins

Associated data

  • PDB/1WDZ