The first case of hyphema occurring during selective laser trabeculoplasty in an eye without neovascularization is described. A 77-year-old man with uncontrolled open-angle glaucoma received selective laser trabeculoplasty in both eyes for high intraocular pressure with maximally tolerated medical treatment. Hyphema occurred during selective laser trabeculoplasty in the left eye. This resolved spontaneously without sequelae. Successful intraocular pressure control was achieved. Hyphema and bleeding can happen during selective laser trabeculoplasty. Although this was transient and uneventful in one patient, careful monitoring of intraocular pressure and anterior chamber reaction is advised.