[Inhibition of serine/threonine kinase 15 gene expression induces apoptosis of gastric cancer cells]

Zhonghua Wei Chang Wai Ke Za Zhi. 2006 Sep;9(5):417-20.
[Article in Chinese]

Abstract

Objective: To observe the effect of inhibition of serine/threonine kinase15 (STK15) gene expression on apoptosis induction in gastric cancer cell line-MKN45 and discuss the role of STK15 in viability of gastric cancer cells.

Methods: The STK15 expression was inhibited by chemically synthesized siRNA. The STK15 mRNA and protein level were respectively measured by real-time quantitative PCR and western blotting,the change of cell cycle distribution and apoptosis rate were detected by flow-cytometry, cell morphological change was observed by Hoechst staining,and pro-caspase 3 level was also detected by western blot.

Results: After treatment by siRNA targeting STK15 after 48 h, STK15 mRNA and protein level decreased obviously. More MKN45 cells accumulated at G(2)/M phase (P< 0.05). The apoptosis rate of STK15 siRNA treated MKN45 cells was higher than that of control cells(P< 0.05) with the pro-caspase 3 level decreased.

Conclusions: Inhibition of STK15 gene expression may induce apoptosis in MKN45 cells through the pathway of caspase3. STK15 gene play a key role in proliferation and viability of MKN45 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis*
  • Aurora Kinase A
  • Aurora Kinases
  • Cell Line, Tumor
  • Gene Expression Regulation, Neoplastic
  • Gene Silencing*
  • Humans
  • Protein Serine-Threonine Kinases / genetics*
  • RNA, Small Interfering
  • Stomach Neoplasms

Substances

  • RNA, Small Interfering
  • AURKA protein, human
  • Aurora Kinase A
  • Aurora Kinases
  • Protein Serine-Threonine Kinases