A lectin-binding soluble factor released by CD8+CD57+ lymphocytes from AIDS patients inhibits T cell cytotoxicity

Eur J Immunol. 1991 Mar;21(3):737-41. doi: 10.1002/eji.1830210329.

Abstract

CD8+CD57+ T cells, expanded in peripheral blood lymphocytes of AIDS patients, inhibit the effector phase of HLA-specific cytotoxic T lymphocytes, natural killer and lymphocyte-activated killer cells in a 4-h chromium-release assay. This inhibitory activity present in supernatants of purified sorted CD8+CD57+ cells is mediated by a non-antigen-specific inhibitory factor which is distinct from prostaglandin E2, T cell growth factor (TGF)-beta, latent-TGF-beta, tumor necrosis factor (TNF)-alpha and TNF-beta. Partial biochemical characterization demonstrates that the CD8+CD57+ inhibitory activity (a) is heat, trypsin and acid resistant, (b) binds to concanavalin A columns, indicating its glycosylation state and (c) is mediated by a 20-30-kDa soluble molecule.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acquired Immunodeficiency Syndrome / immunology*
  • Antigens, Differentiation, T-Lymphocyte / analysis
  • CD57 Antigens
  • CD8 Antigens
  • Cytotoxicity, Immunologic*
  • Humans
  • Immunity, Cellular
  • In Vitro Techniques
  • Lectins / metabolism
  • Molecular Weight
  • Solubility
  • Suppressor Factors, Immunologic / chemistry
  • Suppressor Factors, Immunologic / physiology*
  • T-Lymphocyte Subsets / immunology*
  • T-Lymphocytes, Cytotoxic / immunology*

Substances

  • Antigens, Differentiation, T-Lymphocyte
  • CD57 Antigens
  • CD8 Antigens
  • Lectins
  • Suppressor Factors, Immunologic