Nucleocapsid amino acids 211 to 254, in particular, tetrad glutamines, are essential for the interaction between the nucleocapsid and membrane proteins of SARS-associated coronavirus

J Microbiol. 2006 Oct;44(5):577-80.

Abstract

GST pull-down assays were used to characterize the SARS-CoV membrane (M) and nucleocapsid (N) interaction, and it was found that the amino acids 211-254 of N protein were essential for this interaction. When tetrad glutamines (Q) were replaced with glutamic acids (E) at positions of 240-243 of the N protein, the interaction was disrupted.

MeSH terms

  • Amino Acid Substitution / genetics
  • Binding Sites / genetics
  • Coronavirus Nucleocapsid Proteins
  • Electrophoresis, Polyacrylamide Gel
  • Glutamic Acid / genetics
  • Glutamic Acid / metabolism
  • Glutamine / genetics
  • Glutamine / metabolism*
  • Nucleocapsid / metabolism*
  • Nucleocapsid Proteins / genetics
  • Nucleocapsid Proteins / metabolism*
  • Peptides / genetics
  • Peptides / metabolism
  • Protein Binding / genetics
  • Severe acute respiratory syndrome-related coronavirus / genetics
  • Severe acute respiratory syndrome-related coronavirus / metabolism*

Substances

  • Coronavirus Nucleocapsid Proteins
  • Nucleocapsid Proteins
  • Peptides
  • Glutamine
  • polyglutamine
  • Glutamic Acid