Usefulness of the BACTEC MGIT 960 system for isolation of mycobacterium tuberculosis from sputa subjected to long-term storage

Manuela Pardini; Francis Varaine; Maryline Bonnet; Graziella Orefici; Marco Rinaldo Oggioni; LONG-DRUG Study Group; Lanfranco Fattorini

doi:10.1128/JCM.01985-06

Usefulness of the BACTEC MGIT 960 system for isolation of mycobacterium tuberculosis from sputa subjected to long-term storage

J Clin Microbiol. 2007 Feb;45(2):575-6. doi: 10.1128/JCM.01985-06. Epub 2006 Nov 22.

Authors

Manuela Pardini¹, Francis Varaine, Maryline Bonnet, Graziella Orefici, Marco Rinaldo Oggioni; LONG-DRUG Study Group; Lanfranco Fattorini

Affiliation

¹ Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.

Abstract

The recovery of Mycobacterium tuberculosis from sputa positive or negative for acid-fast bacilli that were stored for 17 +/- 7 days and inoculated in the BACTEC MGIT 960 system (MGIT) was higher than that from sputa inoculated in Lowenstein-Jensen medium. MGIT is useful for isolation of M. tuberculosis from sputa subjected to long-term storage.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacteriological Techniques
Culture Media
Humans
Mycobacterium tuberculosis / isolation & purification*
Reagent Kits, Diagnostic*
Specimen Handling / methods*
Sputum / microbiology*
Time Factors
Tuberculosis, Pulmonary / microbiology*

Substances

Culture Media
Reagent Kits, Diagnostic