Abstract
We used RNAase protection and restriction fragment length polymorphism assays to detect activating mutations of c-src in a spectrum of human tumours. No mutations were detected at codons 98, 381, 444, and 530. We conclude that mutational activation is not the mechanism of enhancement of pp60c-src-specific kinase activity found in a number of human cancer types.
MeSH terms
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Base Sequence
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Codon
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Genes, src*
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Humans
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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Mutation*
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Neoplasms / enzymology
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Neoplasms / genetics*
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Oligodeoxyribonucleotides
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Polymorphism, Restriction Fragment Length
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Protein-Tyrosine Kinases / genetics
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Protein-Tyrosine Kinases / metabolism
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Proto-Oncogene Proteins pp60(c-src) / genetics
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Proto-Oncogene Proteins pp60(c-src) / metabolism*
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RNA, Messenger / genetics
Substances
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Codon
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Oligodeoxyribonucleotides
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RNA, Messenger
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Protein-Tyrosine Kinases
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Proto-Oncogene Proteins pp60(c-src)