Petrotoga halophila sp. nov., a thermophilic, moderately halophilic, fermentative bacterium isolated from an offshore oil well in Congo

Int J Syst Evol Microbiol. 2007 Jan;57(Pt 1):40-44. doi: 10.1099/ijs.0.64516-0.

Abstract

A novel thermophilic, moderately halophilic, rod-shaped bacterium, strain MET-B(T), with a sheath-like outer structure (toga) was isolated from an offshore oil-producing well in Congo, West Africa. Strain MET-B(T) was a Gram-negative bacterium with the ability to reduce elemental sulfur, but not sulfate, thiosulfate or sulfite into sulfide. The optimum growth conditions were 60 degrees C, pH 6.7-7.2 and 4-6 % NaCl. The DNA G+C content was 34.6 mol%. Strain MET-B(T) was phylogenetically related to members of the genus Petrotoga; Petrotoga miotherma, Petrotoga olearia and Petrotoga mexicana were the closest relatives, with type strains exhibiting more than 99 % identity in an analysis of small-subunit rRNA gene sequences. The values for DNA-DNA relatedness between the type strains of these three species and strain MET-B(T) were less than 42 %. As MET-B(T) was found to be genetically and physiologically different from other species of the genus Petrotoga, this strain is proposed as representing a novel species, for which the name Petrotoga halophila sp. nov. is proposed. The type strain is MET-B(T) (=DSM 16923(T)=CCUG 50214(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Congo
  • DNA, Bacterial / analysis
  • DNA, Ribosomal / analysis
  • Genes, rRNA
  • Gram-Negative Anaerobic Straight, Curved, and Helical Rods / classification*
  • Gram-Negative Anaerobic Straight, Curved, and Helical Rods / genetics
  • Gram-Negative Anaerobic Straight, Curved, and Helical Rods / isolation & purification*
  • Gram-Negative Anaerobic Straight, Curved, and Helical Rods / physiology
  • Hot Temperature*
  • Molecular Sequence Data
  • Petroleum*
  • Phenotype
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sodium Chloride*

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Petroleum
  • RNA, Ribosomal, 16S
  • Sodium Chloride

Associated data

  • GENBANK/AY800102