Anti-thyroid-stimulating hormone receptor antibodies determined by second-generation assay

Clin Chem Lab Med. 2007;45(1):26-9. doi: 10.1515/CCLM.2007.016.

Abstract

Background: The aim of our study was to determine the frequency of anti-thyroid-stimulating hormone (TSH) receptor antibodies (TRAb) in Tunisian patients with Graves' disease (GD) and to compare the validity of TRAb to that of thyroperoxidase (TPO-Ab) and thyroglobulin antibodies (TG-Ab).

Methods: ELISA was used to determine the frequency of TRAb, TPO-Ab and TG-Ab in sera of 190 patients with GD. Patients were divided into four groups: those with untreated active GD (group A, n=71), those receiving treatment with anti-thyroid drugs (group B, n=85), those in relapse (group C, n=15) and those in remission (group D, n=19). Sera of 100 healthy blood donors served as controls.

Results: The sensitivity of TRAb for the diagnosis of GD (95.8%) was significantly higher than that of TPO-Ab (73.2%) and TG-Ab (42.2%) (p=0.0005 and p<10(-7), respectively). The positive rate for TRAb was lower in group B than in group A (70.6% and 95.8%, respectively; p=0.0001). The levels of TRAb were significantly higher in group A than in group B (mean 30.1 and 14.2 IU/L, respectively; p=0.006).

Conclusions: TRAb, but neither TPO-Ab nor TG-Ab, is valuable in the diagnosis and management of patients with GD.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Autoantibodies / blood*
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Female
  • Goiter / immunology
  • Goiter / pathology
  • Graves Disease / immunology*
  • Humans
  • Hyperthyroidism / blood
  • Iodide Peroxidase / blood
  • Male
  • Middle Aged
  • ROC Curve
  • Receptors, Thyrotropin / immunology*
  • Sensitivity and Specificity
  • Thyroglobulin / blood
  • Tunisia

Substances

  • Autoantibodies
  • Receptors, Thyrotropin
  • Thyroglobulin
  • Iodide Peroxidase