Oxidation artifacts in the electrospray mass spectrometry of Abeta Peptide

Anal Chem. 2007 Mar 1;79(5):2031-6. doi: 10.1021/ac061743r. Epub 2007 Jan 24.

Abstract

Gradual corrosion of stainless steel electrospray emitters under conditions of normal use generates surface irregularities that can promote electrical discharge. The increased emission current affects the electrochemical reactions associated with the spray process. When sampling the peptide Abeta(1-40), this is manifest by oxidation of methionine at position 35 to methionine sulfoxide. The resultant mass shift and reduced sensitivity can adversely affect H/D exchange experiments. These effects can be avoided by adding a redox buffer or (preferably) by repolishing the emitter, especially to a rounded geometry.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amyloid beta-Peptides / chemistry*
  • Artifacts*
  • Buffers
  • Corrosion
  • Methionine / analogs & derivatives
  • Methionine / analysis
  • Methionine / chemistry*
  • Oxidation-Reduction
  • Oxygen / chemistry
  • Peptide Fragments / chemistry*
  • Spectrometry, Mass, Electrospray Ionization / standards*
  • Stainless Steel / chemistry
  • Surface Properties

Substances

  • Amyloid beta-Peptides
  • Buffers
  • Peptide Fragments
  • amyloid beta-protein (1-40)
  • Stainless Steel
  • Methionine
  • Oxygen
  • methionine sulfoxide