Localization of the single copy gene Mdh2 on Xenopus tropicalis chromosomes by FISH-TSA

Cytogenet Genome Res. 2007;116(1-2):110-2. doi: 10.1159/000097427.

Abstract

A single copy gene, mitochondrial malate dehydrogenase 2 (Mdh2), was localized on Xenopus tropicalis chromosomes by fluorescence in situ hybridization coupled with tyramide signal amplification (FISH-TSA). The respective cDNA was cloned and sequenced. The labeled probe hybridized with a subcentromeric region of the long arms of homologous chromosomes 3. Results of comparison of the gene localization with previously mapped X. laevis paralogs strongly suggest a common evolutionary origin of chromosomes 3 and 8 in X. laevis and chromosome 3 in X. tropicalis. This is the first time that a single copy gene has been visualized on X. tropicalis chromosomes. The FISH-TSA method gives a strong signal with a 1-kb labeled probe.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromosome Mapping / methods*
  • Cloning, Molecular
  • DNA, Complementary / metabolism
  • Genetic Linkage
  • Hybridization, Genetic
  • In Situ Hybridization, Fluorescence*
  • Malate Dehydrogenase / genetics*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization*
  • Sequence Homology, Amino Acid
  • Xenopus / genetics*
  • Xenopus Proteins / genetics*
  • Xenopus laevis

Substances

  • DNA, Complementary
  • Xenopus Proteins
  • Malate Dehydrogenase