The use of functional genomics for the identification of a gene cluster encoding for the biosynthesis of an antifungal tambjamine in the marine bacterium Pseudoalteromonas tunicata

Catherine Burke; Torsten Thomas; Suhelen Egan; Staffan Kjelleberg

doi:10.1111/j.1462-2920.2006.01177.x

The use of functional genomics for the identification of a gene cluster encoding for the biosynthesis of an antifungal tambjamine in the marine bacterium Pseudoalteromonas tunicata

Environ Microbiol. 2007 Mar;9(3):814-8. doi: 10.1111/j.1462-2920.2006.01177.x.

Authors

Catherine Burke¹, Torsten Thomas, Suhelen Egan, Staffan Kjelleberg

Affiliation

¹ School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney 2052, Australia.

PMID: 17298379
DOI: 10.1111/j.1462-2920.2006.01177.x

Abstract

A large insert library was created in Escherichia coli from the DNA of the surface-associated marine bacterium Pseudoalteromonas tunicata. Screening of the library for antifungal activity resulted in the detection and identification of a large gene cluster encoding for the biosynthesis of an antifungal tambjamine. A biosynthetic pathway has been proposed based on analysis and annotation of the gene cluster.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antifungal Agents / metabolism*
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism*
Escherichia coli / genetics
Escherichia coli / metabolism
Gene Expression Regulation, Bacterial
Gene Library
Genomics*
Multigene Family*
Pseudoalteromonas / genetics
Pseudoalteromonas / growth & development
Pseudoalteromonas / metabolism*
Seawater / microbiology*

Substances

Antifungal Agents
Bacterial Proteins