[Characterization of HIV-1 Gag-specific proliferative T cell responses in Chinese HIV-1 infected patients]

Zhonghua Yi Xue Za Zhi. 2006 Nov 28;86(44):3099-103.
[Article in Chinese]

Abstract

Objective: To investigate the characteristics of HIV-1 Gag-specific T lymphocyte proliferative responses in Chinese HIV infected patients, analyze their associations with disease progression and explore the mechanism of the HIV-specific immune dysfunction in HIV infection.

Methods: Specific proliferative responses of PBMC from thirty four HIV-1 infected patients to HIV-1 Gag antigen were detected by a BrdU incorporated flow cytometry intracellular stain, their relations with CD4(+) T cells absolute counts, plasma viral loads and CD8(+) T cell activate status were analyzed.

Results: HIV-1 Gag-specific CD4(+) T cell proliferative responses of HIV-infected patients were significantly lower than that of healthy controls (P < 0.01), correlated inversely to CD38 expression on CD8(+) T lymphocytes and plasma viral loads (P < 0.05, P < 0.05), and directly to CD4(+) T cell absolute counts (P < 0.01). HIV-1 Gag-specific CD8(+) T cell proliferative responses of HIV-infected patients were significantly higher than that of healthy controls (P < 0.01), correlated directly to CD38 expression on CD8(+) T lymphocytes (P < 0.05), and inversely to CD4(+) T cell absolute counts (P < 0.01), but not correlated to viral loads (P > 0.05).

Conclusion: HIV-1 Gag-specific T cell proliferative response were abnormal in Chinese HIV-1 infected persons, HIV-1 Gag-specific CD4(+) T cell proliferative responses were decreased and their frequencies were closely correlated with disease progression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Asian People*
  • CD4-Positive T-Lymphocytes / immunology
  • CD4-Positive T-Lymphocytes / pathology
  • CD4-Positive T-Lymphocytes / virology
  • CD8-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / pathology
  • CD8-Positive T-Lymphocytes / virology
  • Cell Proliferation*
  • China
  • Female
  • Flow Cytometry
  • HIV Infections / blood
  • HIV Infections / ethnology
  • HIV Infections / immunology*
  • HIV-1 / immunology*
  • HIV-1 / metabolism
  • Humans
  • Lymphocyte Activation / immunology
  • Male
  • Middle Aged
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / pathology
  • T-Lymphocytes / virology
  • Viral Load
  • gag Gene Products, Human Immunodeficiency Virus / immunology*
  • gag Gene Products, Human Immunodeficiency Virus / metabolism

Substances

  • gag Gene Products, Human Immunodeficiency Virus