Effect of lysozyme on plasma membranes of liver and heart cells: lectin binding assay

M V Mikaelyan; G G Poghosyan; I E Stepanyan; V K Gasparyan

doi:10.1016/j.cellbi.2007.01.001

Effect of lysozyme on plasma membranes of liver and heart cells: lectin binding assay

Cell Biol Int. 2007 Jul;31(7):699-702. doi: 10.1016/j.cellbi.2007.01.001. Epub 2007 Jan 14.

Authors

M V Mikaelyan¹, G G Poghosyan, I E Stepanyan, V K Gasparyan

Affiliation

¹ Institute of Biochemistry, Physical Chemistry of Proteins, National Academy of Sciences, P.Sevak str. 5/1, Yerevan 375014, Armenia.

PMID: 17317226
DOI: 10.1016/j.cellbi.2007.01.001

Abstract

Lectin binding assay was applied to polyacrylamide slices to study the effects of lysozyme on carbohydrate profile of plasma membranes from liver and heart tissues. The investigations were carried out on polyacrylamide slices immobilized with wheat germ agglutinin or Concanavalin A. It was shown that lysozyme changes the carbohydrate profile of plasma membranes of liver and heart cells.

Publication types

Evaluation Study

MeSH terms

Acrylic Resins / chemistry
Animals
Carbohydrates / analysis
Cell Membrane / chemistry
Cell Membrane / drug effects*
Cell Survival / drug effects
Clinical Laboratory Techniques
Concanavalin A / chemistry
Concanavalin A / metabolism
Heart / drug effects*
Hepatocytes / drug effects*
Hepatocytes / ultrastructure
Lectins / metabolism*
Muramidase / pharmacology*
Myocardium / cytology
Myocardium / ultrastructure
Protein Binding
Rats
Tissue Extracts / chemistry
Wheat Germ Agglutinins / chemistry

Substances

Acrylic Resins
Carbohydrates
Lectins
Tissue Extracts
Wheat Germ Agglutinins
polyacrylamide gels
Concanavalin A
Muramidase