Abstract
In 1997, we developed a PCR assay for the detection of herpes simplex virus (HSV) DNA. Recently, we determined an optimal positivity criterion based on research specimens and a dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-fold lower than our previous cutoff, minimizes misclassification.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
DNA, Viral / genetics*
-
DNA, Viral / isolation & purification*
-
False Positive Reactions
-
Humans
-
Mucous Membrane / virology*
-
Polymerase Chain Reaction / methods*
-
Polymerase Chain Reaction / standards*
-
Reproducibility of Results
-
Sensitivity and Specificity
-
Simplexvirus / genetics*
-
Simplexvirus / isolation & purification*
-
Skin / virology*
-
Virus Shedding