Highly induced DNA recombination mediated by membrane permeabilized recombinant cre protein in mouse primary cells

Biosci Biotechnol Biochem. 2007 Mar;71(3):817-20. doi: 10.1271/bbb.60623. Epub 2007 Mar 7.

Abstract

To establish efficient induction of Cre mediated DNA recombination in primary cells, mouse embryonic fibroblast, keratinocyte, and primary preosteoblast, we tested various recombinant Cres by fusing of protein transduction domain in human immunodeficiency virus (HIV) transactivator of transcription (TAT-PTD) to the N- and/or C-terminus. HTC, modified Cre with PTD at the N-terminus, achieved the highest activity of DNA recombination for those primary cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques
  • Cell Membrane Permeability / physiology*
  • Cells, Cultured
  • DNA / metabolism*
  • Embryo, Mammalian / cytology
  • Fibroblasts / metabolism
  • Gene Products, tat*
  • Integrases / genetics
  • Integrases / metabolism*
  • Keratinocytes / metabolism*
  • Mice
  • Osteoblasts / metabolism
  • Protein Structure, Tertiary / genetics
  • Protein Structure, Tertiary / physiology
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Recombination, Genetic / genetics
  • Recombination, Genetic / physiology*
  • beta-Galactosidase / metabolism

Substances

  • Gene Products, tat
  • Recombinant Fusion Proteins
  • DNA
  • Cre recombinase
  • Integrases
  • beta-Galactosidase