Development of new-generation HU-PBMC-NOD/SCID mice to study human islet alloreactivity

Ann N Y Acad Sci. 2007 Apr:1103:90-3. doi: 10.1196/annals.1394.011. Epub 2007 Mar 21.

Abstract

The use of "humanized" mice represents an appealing translational model for studies of the pathogenesis of immune-mediated diseases and for the evaluation of potential therapeutics. The utility of humanized mice depends on their ability to model the human immune system with high fidelity, and, in this respect, previous models have fallen short. The recently developed NOD-scid Il2rgamma(null) mouse, however, exhibits greatly enhanced ability to support the engraftment of human peripheral blood mononuclear cells. Herein, we describe the challenges of recapitulating human immunity in humanized mice and features of NOD-scid Il2rgamma(null) mice that help overcome them.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Crosses, Genetic
  • Diabetes Mellitus, Type 1 / immunology*
  • Disease Models, Animal
  • Humans
  • Islets of Langerhans / immunology*
  • Isoantigens / immunology
  • Leukocytes, Mononuclear / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred NOD / genetics*
  • Mice, SCID / genetics*

Substances

  • Isoantigens