Tenascin-W inhibits proliferation and differentiation of preosteoblasts during endochondral bone formation

Biochem Biophys Res Commun. 2007 May 18;356(4):935-41. doi: 10.1016/j.bbrc.2007.03.071. Epub 2007 Mar 22.

Abstract

We identified a cDNA encoding mouse Tenascin-W (TN-W) upregulated by bone morphogenetic protein (Bmp)2 in ATDC5 osteo-chondroprogenitors. In adult mice, TN-W was markedly expressed in bone. In mouse embryos, during endochondral bone formation TN-W was localized in perichondrium/periosteum, but not in trabecular and cortical bones. During bone fracture repair, cells in the newly formed perichondrium/periosteum surrounding the cartilaginous callus expressed TN-W. Furthermore, TN-W was detectable in perichondrium/periosteum of Runx2-null and Osterix-null embryos, indicating that TN-W is expressed in preosteoblasts. In CFU-F and -O cells, TN-W had no effect on initiation of osteogenesis of bone marrow cells, and in MC3T3-E1 osteoblastic cells TN-W inhibited cell proliferation and Col1a1 expression. In addition, TN-W suppressed canonical Wnt signaling which stimulates osteoblastic differentiation. Our results indicate that TN-W is a novel marker of preosteoblasts in early stage of osteogenesis, and that TN-W inhibits cell proliferation and differentiation of preosteoblasts mediated by canonical Wnt signaling.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Bone Development / drug effects
  • Bone Development / physiology*
  • Cell Differentiation / physiology
  • Cell Proliferation
  • Embryonic Stem Cells / cytology*
  • Embryonic Stem Cells / physiology*
  • Mice
  • Mice, Inbred ICR
  • Osteoblasts / cytology*
  • Osteoblasts / physiology*
  • Osteogenesis / physiology
  • Tenascin / metabolism*
  • Wnt Proteins / metabolism*
  • Zebrafish Proteins / metabolism*

Substances

  • Tenascin
  • Wnt Proteins
  • Zebrafish Proteins
  • tnn protein, zebrafish