Abstract
By hybridizing human adult testis cDNA microarrays with human adult and embryo testis cDNA probes, we identified a novel human testis gene, PINCH 2. PINCH 2 expression was 3.4-fold higher in adult than in fetal testis. The full length of its cDNA was 963 bp, with a 354-bp open reading frame (ORF), encoding a 117-amino acid protein. PINCH 2 was a splicing isoform of PINCH. It shared one exon, which encoded the LIM domain, with PINCH gene in human genome. Multitissue reverse transcriptase-polymerase chain reaction (RTPCR) analysis revealed that this gene was expressed variably in a wide range of tissues, with high expression levels in human adult testis. These results suggest that PINCH 2, a novel LIM domain-containing gene, may play an important role in testicular development/spermatogenesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Alternative Splicing
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Amino Acid Sequence
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Base Sequence
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Biotechnology
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Cloning, Molecular
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DNA Primers / genetics
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DNA, Complementary / genetics
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Female
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Gene Expression Profiling
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Genetic Variation
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Humans
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LIM Domain Proteins
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Male
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Membrane Proteins
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Molecular Sequence Data
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Multigene Family
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Oligonucleotide Array Sequence Analysis
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Pregnancy
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Homology, Amino Acid
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Spermatogenesis / genetics
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Testis / embryology
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Testis / growth & development
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Testis / metabolism*
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Tissue Distribution
Substances
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Adaptor Proteins, Signal Transducing
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DNA Primers
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DNA, Complementary
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DNA-Binding Proteins
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LIM Domain Proteins
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LIMS2 protein, human
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Membrane Proteins
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Protein Isoforms
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RNA, Messenger