The chloroxoquinolinic derivative 6-chloro-1,4-dihydro-4-oxo-1-(beta-D-ribofuranosyl) quinoline-3-carboxylic acid inhibits HSV-1 adsorption by impairing its adsorption on HVEM

Arch Virol. 2007;152(7):1417-24. doi: 10.1007/s00705-007-0960-y. Epub 2007 Apr 26.

Abstract

In this paper, we describe that the oxoquinolinic acid derivative (compound A) inhibited HSV-1 adsorption on Vero cells. This effect was achieved with an EC(50) value of 10 +/- 2.0 microM and with low cytotoxicity, since the CC(50) value for compound A was >1000 microM. Moreover, we demonstrate for the first time that adsorption inhibition was due to the blockage of the interactions between HSV-1 and the cellular receptor herpes virus entry mediator (HVEM). These results show that compound A can prevent HSV-1 infection in Vero cells, encouraging further studies to determine at what level compound A inhibits HSV-1-HVEM interactions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyclovir / pharmacology
  • Adsorption
  • Animals
  • Antiviral Agents / chemistry
  • Antiviral Agents / pharmacology
  • CHO Cells
  • Chlorocebus aethiops
  • Cricetinae
  • Cricetulus
  • Herpesvirus 1, Human / drug effects*
  • Herpesvirus 1, Human / pathogenicity
  • In Vitro Techniques
  • Quinolones / chemistry
  • Quinolones / pharmacology*
  • Receptors, Tumor Necrosis Factor, Member 14 / drug effects*
  • Receptors, Tumor Necrosis Factor, Member 14 / physiology
  • Vero Cells

Substances

  • Antiviral Agents
  • Quinolones
  • Receptors, Tumor Necrosis Factor, Member 14
  • Acyclovir