Two-dimensional electrophoresis of 1D-encoded B and D glutenin subunits in common wheats with similar omega gliadins

Biochem Genet. 1991 Aug;29(7-8):403-13. doi: 10.1007/BF00554146.

Abstract

Gli-D1-encoded omega gliadins of bread wheats show little variation; their electrophoretic patterns can be classified into two main groups which broadly resemble the patterns found in the cultivars Chinese Spring and in Cheyenne. B and D subunits of low molecular weight glutenin encoded by the chromosome 1D loci Glu-D3 and Gli-D1, respectively, also showed little variation. D subunits were found only in bread wheats with "Chinese Spring-type" omega gliadins and they all exhibited the same electrophoretic pattern. This material also showed very similar B subunits. "Cheyenne-type" bread wheats displayed the same electrophoretic distribution of chromosome 1D-encoded B subunits, although they were slightly different from that found in Cheyenne itself.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrophoresis, Gel, Two-Dimensional
  • Genetic Variation
  • Gliadin / chemistry*
  • Gliadin / genetics
  • Glutens / analogs & derivatives*
  • Glutens / chemistry
  • Glutens / genetics
  • Molecular Weight
  • Protein Conformation
  • Species Specificity
  • Triticum / chemistry*
  • Triticum / genetics

Substances

  • Glutens
  • Gliadin
  • glutenin