Abstract
The authors have demonstrated a strong T-cell inhibitory activity of hepatic stellate cells (HSC), which may participate in the establishment of hepatic tolerance. The underlying mechanism is not completely understood. This study showed that intercellular adhesion molecule 1 (ICAM-1) was constitutively expressed on HSC, and up-regulated upon activation. ICAM-1 knockout mice was used to analyze the role of ICAM-1 expressed on HSC, and showed that deficiency in ICAM-1 expression partially reverses HSC immune inhibitory activity both in vitro and in vivo, but did not significantly affect their capacity to induce T-cell apoptosis.
Copyright (c) 2007 Wiley-Liss, Inc. Microsurgery 2007.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Annexin A5 / metabolism
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Apoptosis
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Cell Culture Techniques
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Diabetes Mellitus, Experimental / immunology
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Flow Cytometry / methods
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Graft Survival
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Hepatocytes / immunology
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Hepatocytes / transplantation*
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Intercellular Adhesion Molecule-1 / genetics
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Intercellular Adhesion Molecule-1 / immunology*
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Intercellular Adhesion Molecule-1 / metabolism
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Islets of Langerhans Transplantation / immunology*
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Islets of Langerhans Transplantation / methods
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Islets of Langerhans Transplantation / pathology
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Male
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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T-Lymphocytes / immunology*
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Time Factors
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Transplantation, Homologous
Substances
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Annexin A5
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Intercellular Adhesion Molecule-1