Recruitment of DNA damage recognition and repair pathway proteins following near-IR femtosecond laser irradiation of cells

J Biomed Opt. 2007 Mar-Apr;12(2):020505. doi: 10.1117/1.2717684.

Abstract

An 800-nm 200-fs laser is used to produce DNA damage in rat kangaroo (PtK1) and human cystic fibrosis pancreatic adenoma carcinoma (CFPAC-1) cells. Immunofluorescence staining for DNA repair factors in irradiated cells displays localization of gammaH2AX, Nbs1, and Rad50 to the site of irradiation 3 to 30 min following laser exposure. It is concluded that the 200-fs near-infrared laser is an excellent source for the production and study of spatially defined regions of DNA damage.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / chemistry
  • DNA / genetics*
  • DNA / radiation effects*
  • DNA Damage*
  • DNA-Binding Proteins / genetics*
  • Dose-Response Relationship, Radiation
  • Infrared Rays*
  • Lasers*
  • Radiation Dosage

Substances

  • DNA-Binding Proteins
  • DNA