Abstract
We developed a system for rapid determination of viral RNA sequences whereby genomic sequence is obtained from cultured virus isolates without subcloning into plasmid vectors. This method affords new opportunities to address the challenges of unknown or untypeable emerging viruses.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Base Sequence
-
DNA, Complementary / genetics
-
Genome, Viral / genetics*
-
Genomics / methods*
-
Nucleic Acid Amplification Techniques / methods*
-
RNA Viruses / genetics*
-
RNA, Viral / genetics*
-
Time Factors
Substances
-
DNA, Complementary
-
RNA, Viral