Formation of an aminoacyl-S-enzyme intermediate is a key step in the biosynthesis of chloramphenicol

Michelle Pacholec; Jason K Sello; Christopher T Walsh; Michael G Thomas

doi:10.1039/b703356g

Formation of an aminoacyl-S-enzyme intermediate is a key step in the biosynthesis of chloramphenicol

Org Biomol Chem. 2007 Jun 7;5(11):1692-4. doi: 10.1039/b703356g. Epub 2007 Apr 26.

Authors

Michelle Pacholec¹, Jason K Sello, Christopher T Walsh, Michael G Thomas

Affiliation

¹ Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.

PMID: 17520135
DOI: 10.1039/b703356g

Abstract

Herein we report the first biochemical characterization of an enzyme involved in the biosynthesis of chloramphenicol that provides new insights into the origins of the antibiotic.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Chloramphenicol / biosynthesis*
Chloramphenicol / chemistry
Kinetics
NADH, NADPH Oxidoreductases / metabolism*
Streptomyces / enzymology*
Time Factors

Substances

Chloramphenicol
NADH, NADPH Oxidoreductases