Gas-phase fragmentation of protonated benzodiazepines

Rapid Commun Mass Spectrom. 2007;21(14):2273-81. doi: 10.1002/rcm.3084.

Abstract

Protonated 1,4-benzodiazepines dissociate in the gas phase by the common pathway of CO elimination and by unique pathways dictated by the substituents; the latter typically differentiate one benzodiazepine from another. Protonated 3-dihydro-5-phenyl-1,4-benzodiazepin-2-one, the base diazepam devoid of substituents, dissociates by eliminating CO, HNCO, benzene, and benzonitrile. Mechanisms of these reactions are proposed with ionic products being resonance stabilized. The abundant [MH-CO]+ ion dissociates to secondary products via elimination of benzene, benzonitrile, the NH2 radical, and ammonia, yielding again ionic products that are stabilized by resonance.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzodiazepines / analysis
  • Benzodiazepines / chemistry*
  • Drug Evaluation, Preclinical / methods
  • Gases / analysis
  • Gases / chemistry*
  • Phase Transition
  • Protons
  • Spectrometry, Mass, Electrospray Ionization / methods*

Substances

  • Gases
  • Protons
  • Benzodiazepines