In this paper, the definitions of soil macropore and macropore flow, their types, characteristics, generation mechanisms, and experimental methods in laboratory and field were introduced, and the research progress in their determination techniques, including dye tracing, slice up, penetration curve, tension infiltration apparatus, X-ray CT scanning, and radar exploration were summarized. It was indicated that because of the complexity of the factors affecting soil macropore and macropore flow, the experiments in laboratory should be combined with those in field, and the observation techniques and simulation measures should be further improved.