Abstract
Replication protein A (RPA) is a stable heterotrimeric complex consisting of p70, p32 and p14 subunits. The protein plays a crucial role in SV40 minichromosome replication. Peptides of p70 representing interaction sites for the smaller two subunits, DNA as well as the viral initiator protein large T-antigen (Tag) and the cellular DNA polymerase alpha-primase (Pol) all interfered with the replication process indicating the importance of the different p70 activities in this process. Inhibition by the peptide disrupting protein-protein interactions was observed only during the pre-initiation stage prior to primer synthesis, suggesting the formation of a stable initiation complex between RPA, Tag and Pol at the primer end.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Viral, Tumor / genetics
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Antigens, Viral, Tumor / metabolism
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Cell Line
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DNA Polymerase I / genetics
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DNA Polymerase I / metabolism
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DNA Primase / genetics
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DNA Primase / metabolism
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DNA Primers / genetics
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DNA Primers / metabolism
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DNA Replication / physiology*
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DNA, Viral / genetics
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DNA, Viral / metabolism*
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Humans
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Multiprotein Complexes / genetics
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Multiprotein Complexes / metabolism
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Protein Binding / physiology
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Protein Subunits / genetics
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Protein Subunits / metabolism
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Replication Protein A / genetics
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Replication Protein A / metabolism*
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Simian virus 40 / physiology*
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Viral Proteins / genetics
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Viral Proteins / metabolism*
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Virus Replication / physiology*
Substances
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Antigens, Viral, Tumor
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DNA Primers
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DNA, Viral
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Multiprotein Complexes
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Protein Subunits
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Replication Protein A
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Viral Proteins
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DNA Primase
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DNA polymerase alpha-primase
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DNA Polymerase I