Assays of T-cell contact dependent monocyte-macrophage functions

Methods Mol Med. 2007:136:139-48. doi: 10.1007/978-1-59745-402-5_10.

Abstract

The production of cytokines and other inflammatory products in chronic/sterile inflammatory disorders such as rheumatoid arthritis might be induced in monocytes/macrophages by direct cellular contact with stimulated T-cells. Studies of cell-cell interactions are usually complicated by the simultaneous presence of at least two viable cell types. To obviate this problem, we developed strategies allowing only interactions between stimulated T-cell surface molecules and the monocytes/macrophages, and any products secreted into the medium or mRNA are necessarily of monocytic origin. This chapter aims at reviewing and describing the latter methods and strategies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Separation / methods*
  • Cytokines / immunology
  • Humans
  • Lymphocyte Activation
  • Macrophages / cytology
  • Macrophages / immunology*
  • Monocytes / cytology
  • Monocytes / immunology*
  • RNA, Messenger / analysis
  • T-Lymphocytes / cytology
  • T-Lymphocytes / immunology*

Substances

  • Cytokines
  • RNA, Messenger