Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure

Leen Baert; Christiane E Wobus; Els Van Coillie; Larissa B Thackray; Johan Debevere; Mieke Uyttendaele

doi:10.1128/AEM.01039-07

Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure

Appl Environ Microbiol. 2008 Jan;74(2):543-6. doi: 10.1128/AEM.01039-07. Epub 2007 Nov 16.

Authors

Leen Baert¹, Christiane E Wobus, Els Van Coillie, Larissa B Thackray, Johan Debevere, Mieke Uyttendaele

Affiliation

¹ Ghent University, Faculty of Bioscience Engineering, Department of Food Safety and Food Quality, Laboratory of Food Microbiology and Food Preservation, Coupure links 653, 9000 Ghent, Belgium.

Abstract

The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80 degrees C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Caliciviridae Infections / virology
Gastroenteritis / virology
Hot Temperature*
Mice
Norovirus / genetics*
Norovirus / growth & development
RNA, Viral / genetics
RNA, Viral / metabolism
Reverse Transcriptase Polymerase Chain Reaction / methods*
Temperature
Transfection / methods*
Viral Plaque Assay / methods*

Substances

RNA, Viral