Abstract
Both limiting and toxic soil concentrations of the essential micronutrient boron represent major limitations to crop production worldwide. We identified Bot1, a BOR1 ortholog, as the gene responsible for the superior boron-toxicity tolerance of the Algerian barley landrace Sahara 3771 (Sahara). Bot1 was located at the tolerance locus by high-resolution mapping. Compared to intolerant genotypes, Sahara contains about four times as many Bot1 gene copies, produces substantially more Bot1 transcript, and encodes a Bot1 protein with a higher capacity to provide tolerance in yeast. Bot1 transcript levels identified in barley tissues are consistent with a role in limiting the net entry of boron into the root and in the disposal of boron from leaves via hydathode guttation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Biological Transport
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Boron / metabolism
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Boron / toxicity*
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Boron Compounds / metabolism*
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Boron Compounds / toxicity*
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Chromosome Mapping
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Genes, Plant*
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Hordeum / drug effects*
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Hordeum / genetics*
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Hordeum / metabolism
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Membrane Transport Proteins / chemistry
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Membrane Transport Proteins / genetics*
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Membrane Transport Proteins / metabolism
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Molecular Sequence Data
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Plant Lectins / genetics
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Plant Lectins / metabolism
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Plant Proteins / chemistry
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Plant Proteins / genetics
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Plant Proteins / metabolism
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Plant Roots / genetics
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Plant Roots / metabolism
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Quantitative Trait Loci
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae / metabolism
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Transcription, Genetic
Substances
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Boron Compounds
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Membrane Transport Proteins
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Plant Lectins
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Plant Proteins
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Boron