The effectiveness of micronized wheat fibres (MWF) alone or in association with yeast cell walls (YCW) as active adsorbents to decrease, in vivo, the levels of ochratoxin A (OTA) was checked in a total of 48 rats, equitably distributed into four groups: (1) control; (2) OTA naturally contaminated diet (2.2 microg/g); (3) OTA naturally contaminated diet (2.2 microg/g) amended with MWF (2%); (4) OTA naturally contaminated diet (2 microg/g) amended with MWF (1.8%) in association with YCW (0.2%). A 4 week experimental period corresponding to a daily intake in the range of 132.2-146.1 microg OTA/kg bw decreased the rat body weight gains, as compared to the controls. The adsorbents did not significantly alleviate the growth depression caused by the contaminated diet. However, a significant protective effect of MWF was observed in terms of OTA concentration in plasma (40.5% decrease), kidney (28.1% decrease) and liver (38.8% decrease). Mixing this sorbent with the YCW did not significantly improve its protective activity against OTA. The faecal OTA concentrations were higher for the MWF and MWF+YCW treated animals, as compared to the positive control (group II). Taken together, these results suggest that MWF are a promising tool to counteract the toxic effects of OTA naturally contaminated diets.