Glycopolydiacetylene nanoparticles as a chromatic biosensor to detect Shiga-like toxin producing Escherichia coli O157:H7

Bioorg Med Chem Lett. 2008 Jan 15;18(2):700-3. doi: 10.1016/j.bmcl.2007.11.055. Epub 2007 Nov 21.

Abstract

Shiga toxin-producing Escherichia coli organisms (STEC) were detected by Gal-alpha1,4-Gal glycopolydiacetylene (GPDA) nanoparticles through the selective binding between Shiga toxin and GPDA nanoparticles. The binding produced a colorimetric change in the absorption wavelength of the GPDA nanoparticles. This method provides a highly selective, rapid, sensitive, and quantitative approach for the detection of STEC.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques*
  • Escherichia coli O157 / isolation & purification*
  • Escherichia coli O157 / metabolism
  • Nanoparticles*
  • Polyacetylene Polymer
  • Polymers
  • Polyynes
  • Shiga Toxins / biosynthesis*

Substances

  • Polyacetylene Polymer
  • Polymers
  • Shiga Toxins
  • Polyynes
  • polydiacetylene