Ovine atrial natriuretic factor: sequence of circulating forms and metabolism in plasma

Peptides. 1991 Mar-Apr;12(2):279-83. doi: 10.1016/0196-9781(91)90012-e.

Abstract

The sequence of ovine ANF is not known, yet sheep have been used extensively for ANF studies. We sequenced the circulating form of ovine ANF from coronary sinus plasma of sheep in paced heart failure. The main circulating form was identical to human ANF(99-126). Small amounts of ANF identical to human ANF(103-126) and ANF(101-126) peptides were also found. Incubation of labeled ANF in ovine serum suggested ANF(103-126) could be a degradation product of ANF(99-126). The endopeptidase-24.11 degradation product ANF(99-105/106-126) was not found in ovine plasma, in contrast to human plasma where it was a minor component. These results show that while the main circulating forms are similar in sheep and humans, there are differences in the minor peptides.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Atrial Natriuretic Factor / blood*
  • Atrial Natriuretic Factor / chemistry
  • Female
  • Humans
  • Molecular Sequence Data
  • Sequence Homology, Nucleic Acid
  • Species Specificity

Substances

  • Atrial Natriuretic Factor