Urinary 8-isoprostane and 8-OHdG concentrations in boilermakers with welding exposure

J Occup Environ Med. 2008 Feb;50(2):182-9. doi: 10.1097/JOM.0b013e31815cf6cc.

Abstract

Objective: To investigate the association of exposure to fine particulate matter (PM2.5) with DNA damage and oxidative stress in boilermakers exposed to welding fumes.

Methods: Forty-one workers were monitored over 24 hours during which baseline, postshift, bedtime, and next morning measurements were collected. Twenty-two workers participated as controls.

Results: Linear regression was used to model pairwise change in u-8-isoprostane and u-8-OHdG: pre- to postshift, preshift to bedtime, postshift to bedtime, and postshift to next morning. In the models, pre- to postshift change in 8-OHdG was statistically significant, whereas postshift to bedtime change in 8-isoprostane showed an unexpected inverse relationship with PM2.5.

Conclusions: Acute welding exposure is associated with a postshift blunting of systemic inflammation in chronically exposed boilermakers, as measured by 8-isoprostane. The level of oxidative DNA damage as measured by 8-OHdG is less clear.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Adult
  • Air Pollutants, Occupational / adverse effects*
  • Air Pollutants, Occupational / analysis
  • Air Pollution, Indoor / adverse effects
  • Biomarkers / urine
  • DNA Damage
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / urine
  • Dinoprost / analogs & derivatives*
  • Dinoprost / urine
  • Humans
  • Linear Models
  • Massachusetts / epidemiology
  • Middle Aged
  • Occupational Exposure / adverse effects*
  • Oxidative Stress
  • Particulate Matter / urine
  • Prospective Studies
  • Smoking / epidemiology
  • Spirometry
  • Welding*

Substances

  • Air Pollutants, Occupational
  • Biomarkers
  • Particulate Matter
  • 8-epi-prostaglandin F2alpha
  • 8-Hydroxy-2'-Deoxyguanosine
  • Dinoprost
  • Deoxyguanosine