Determination of anti-cyclic citrullinated peptide antibodies in the sera of patients with liver diseases

Clin Exp Rheumatol. 2008 Jan-Feb;26(1):121-4.

Abstract

Objective: To determine the frequency of anti-cyclic citrullinated peptide (anti-CCP) antibodies in patients with HCV infection, primary biliary cirrhosis (PBC) and type-I autoimmune hepatitis (AIH) to assess the specificity of anti-CCP antibodies.

Methods: Rheumatoid factor (RF) and anti-CCP antibodies were measured in the sera from patients with HCV infection (n=45), PBC (n=73), AIH (n=55) and rheumatoid arthritis (n=48), and also from the sera of healthy subjects (n=23). Anti-CCP antibodies were measured using a second generation enzyme-linked immunosorbent assay (ELISA).

Results: No sera with elevated anti-CCP were found in the patients with HCV infection. Two PBC patients (2.7%) and six AIH patients (10.5%) had anti-CCP antibodies. The seropositivity for anti-CCP in these autoimmune disease patients was associated with a high frequency of RA association [PBC; 100% (2/2), AIH; 86.4% (5/6)].

Conclusions: Although anti-CCP antibodies may be present in patients with autoimmune liver diseases, almost seropositive patients had concomitant RA. As a result, the measurement of anti-CCP antibodies may therefore be helpful for accurately diagnosing RA in patients with these liver diseases.

MeSH terms

  • Antibody Specificity
  • Arthritis, Rheumatoid / diagnosis
  • Arthritis, Rheumatoid / immunology
  • Autoantibodies / analysis*
  • Enzyme-Linked Immunosorbent Assay
  • Hepatitis C / immunology
  • Hepatitis, Autoimmune / immunology
  • Humans
  • Liver Cirrhosis, Biliary / immunology
  • Liver Diseases / complications
  • Liver Diseases / immunology*
  • Peptides, Cyclic / immunology*
  • Rheumatoid Factor / analysis

Substances

  • Autoantibodies
  • Peptides, Cyclic
  • cyclic citrullinated peptide
  • Rheumatoid Factor