Bletilla striata polysaccharide stimulates inducible nitric oxide synthase and proinflammatory cytokine expression in macrophages

J Biosci Bioeng. 2008 Feb;105(2):85-9. doi: 10.1263/jbb.105.85.

Abstract

Bletilla striata, a traditional Chinese medicine, has been used for the treatment of alimentary canal mucosal damage, ulcers, bleeding, bruises and burns. B. striata polysaccharide (BSP) isolated from B. striata was found to enhance vascular endothelial cell (EC) proliferation and vascular endothelial growth factor (VEGF) expression. However, the wound healing mechanism of BSP is not well understood. In this study, the results show that treatment with BSP induces coordinate changes in inducible nitric oxide synthase (iNOS), tumor necrosis factor alpha (TNF-alpha) and interleukin 1 beta (IL-1beta) mRNA levels and enhances the expression of these cytokines, but has no effect on interferon gamma (IFN-gamma) level. In this study, we partially elucidate the wound healing mechanism of BSP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cytokines / immunology*
  • Drugs, Chinese Herbal / administration & dosage
  • Gene Expression / drug effects
  • Gene Expression / immunology
  • Immunologic Factors / immunology*
  • Macrophages / drug effects
  • Macrophages / immunology*
  • Mice
  • Nitric Oxide Synthase Type II / immunology*
  • Orchidaceae / metabolism*
  • Polysaccharides / administration & dosage*
  • Wound Healing / immunology*
  • Wound Healing / radiation effects

Substances

  • Cytokines
  • Drugs, Chinese Herbal
  • Immunologic Factors
  • Polysaccharides
  • Nitric Oxide Synthase Type II
  • Nos2 protein, mouse