A trifunctional enzyme with glutathione S-transferase, glutathione peroxidase and superoxide dismutase activity

Biochim Biophys Acta. 2008 Jun;1780(6):869-72. doi: 10.1016/j.bbagen.2008.03.003. Epub 2008 Mar 13.

Abstract

Superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione S-transferase (GST) and glutathione reductase (GR) play crucial roles in balancing the production and decomposition of reactive oxygen species (ROS) in living organisms. These enzymes act cooperatively and synergistically to scavenge ROS, as not one of them can singlehandedly clear all forms of ROS. In order to imitate the synergy of the enzymes, we designed and generated a recombinant protein, which comprises of a Schistosoma japonicum GST (SjGST) and a bifunctional 35-mer peptide with SOD and GPX activities. The engineered protein demonstrated SOD, GPX and GST activities simultaneously. This trifunctional enzyme with SOD, GPX and GST activities is expected to be the best ROS scavenger.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Glutathione Peroxidase / chemistry
  • Glutathione Peroxidase / genetics*
  • Glutathione Transferase / chemistry*
  • Glutathione Transferase / genetics
  • Helminth Proteins / chemistry*
  • Helminth Proteins / genetics
  • Reactive Oxygen Species / chemistry
  • Recombinant Fusion Proteins / chemistry*
  • Recombinant Fusion Proteins / genetics
  • Schistosoma japonicum / enzymology*
  • Schistosoma japonicum / genetics
  • Superoxide Dismutase / chemistry*
  • Superoxide Dismutase / genetics

Substances

  • Helminth Proteins
  • Reactive Oxygen Species
  • Recombinant Fusion Proteins
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione Transferase