Differentiating proteomic biomarkers in breast cancer by laser capture microdissection and MALDI MS

J Proteome Res. 2008 Apr;7(4):1500-7. doi: 10.1021/pr7008109. Epub 2008 Apr 4.

Abstract

We assessed proteomic patterns in breast cancer using MALDI MS and laser capture microdissected cells. Protein and peptide expression in invasive mammary carcinoma versus normal mammary epithelium and estrogen-receptor positive versus estrogen-receptor negative tumors were compared. Biomarker candidates were identified by statistical analysis and classifiers were developed and validated in blinded test sets. Several of the m/ z features used in the classifiers were identified by LC-MS/MS and two were confirmed by immunohistochemistry.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Algorithms
  • Biomarkers, Tumor / analysis*
  • Breast Neoplasms / diagnosis
  • Breast Neoplasms / metabolism*
  • Calgranulin A / analysis
  • Cell Cycle Proteins / analysis
  • Diagnosis, Differential
  • Female
  • Humans
  • Immunohistochemistry
  • Lasers
  • Microdissection / methods*
  • Middle Aged
  • Proteomics / methods*
  • Receptors, Estrogen / analysis
  • Reproducibility of Results
  • S100 Calcium Binding Protein A6
  • S100 Proteins / analysis
  • Sensitivity and Specificity
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Ubiquitin / analysis

Substances

  • Biomarkers, Tumor
  • Calgranulin A
  • Cell Cycle Proteins
  • Receptors, Estrogen
  • S100 Calcium Binding Protein A6
  • S100 Proteins
  • Ubiquitin
  • S100A6 protein, human