Isoenzymes of hexokinase, phosphoglucomutase and glucosephosphate isomerase were examined, using agarose gel isoelectrofocusing, in populations of Phlebotomus ariasi (France), P. neglectus (Greece), P. perfilewi (Italy & Greece) and P. tobbi (Greece). Comparison between enzymatic profiles by numerical analysis separated Larroussius from Phlebotomus and Paraphlebotomus subgenera and brought together P. ariasi and P. neglectus. Nei genetic coefficients indicated similarities between populations of P. perfiliewi from Corfu and central Italy and between Corfuan and Cretan P. neglectus samples.