In a retrospective study, one thousand and ten miscellaneous samples coming from infected newborns and tested at the bacteriology laboratory of A. Royer Children's Hospital from 1983 to 1991 were reviewed. These samples included 471 blood cultures, 114 pus of various origins, 410 cerebrospinal fluids and 15 urines. One bacterium or bacterial soluble antigens was detected in 294 samples (29.2%). Positivity percentage was 29.2% for septicemias, 68% for suppurations, 17.8% for meningitidis and 33.3% for urinary tract infections. There major species were identified: S. aureus (25.8%), Klebsiella spp (19.7%) and E. Coli (14.6%). Altogether, we isolated 156 enterobacteria (53%), 14 Gram negative bacilli (4.7%) and 124 cocci (42.1%) among which 19 streptococci (A, B, C) and 25 pneumococci. The most efficient antibiotics against all strains were AKN, CTX, CRO and GEN.