PIIANP and HELIXII diurnal variation

Osteoarthritis Cartilage. 2008 Oct;16(10):1192-5. doi: 10.1016/j.joca.2008.03.006. Epub 2008 Apr 22.

Abstract

Objective: Serum levels of procollagen type IIA N-terminal propeptide (sPIIANP) and type-II collagen helical peptide (sHELIXII) biomarkers were evaluated for variation diurnally and with physical activity and food in participants with osteoarthritis (OA) of the knee.

Methods: Forty participants with OA of at least one knee were admitted overnight to the General Clinical Research Center for serial serum sampling. Samples were obtained on the evening (6-8 pm) of Day 1 (T3, n=40); prior to rising (8 am) from bed (T0, n=40); 1 h after rising (9 am) without food consumption (T1a, n=20); 1-2 h after rising (9-10 am) with food consumption (T1, n=40); and additionally at noon, 4 h after rising (T2, n=20). sPIIANP and sHELIXII were measured by enzyme-linked immunosorbent assay. Results were analyzed using non-parametric Freidman's test with Dunn's post-hoc multiple comparison test.

Results: Normalized mean concentrations for sPIIANP and sHELIXII increased significantly from T0 to T1 (P<0.05).

Conclusions: This is the first study to demonstrate diurnal variation of these collagen-II biomarkers in individuals with knee OA. These results suggest that serum sampling for these markers should be standardized for purposes of clinical trials.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers / blood
  • Biomarkers / metabolism
  • Cartilage, Articular / pathology*
  • Circadian Rhythm
  • Cohort Studies
  • Collagen Type II / blood
  • Collagen Type II / metabolism*
  • Female
  • Humans
  • Male
  • Middle Aged
  • Osteoarthritis, Knee / blood
  • Osteoarthritis, Knee / pathology*
  • Peptide Fragments / blood
  • Peptide Fragments / metabolism*
  • Statistics as Topic

Substances

  • Biomarkers
  • Collagen Type II
  • Peptide Fragments